Video footage posted on social media showed the star jump to the defence of those who wanted to stand during the show at Melbourne's Ethiad Stadium.
After performing her single Water Under The Bridge, Adele told the staff member: "Excuse me sir? I know you work here but could you stop telling people to sit down? This is a music show.
"If people can't see then they can stand up.
"If you're moaning about people dancing, what the f*** have you come to a show for?
"If I see one more person be told to sit down, I swear to God."
The 77,000-strong crowd also witnessed a surprise proposal when Adele invited a gay couple to join her on stage.