The blast killed 36 militants as it destroyed three underground tunnels as well as weapons and ammunition, a spokesman for the Afghan Ministry of Defense said.
No civilians were hurt, he added.
U.S. forces used a 30-foot long, GPS-guided GBU-43 bomb, at around 7.30pm local time in the Nangarhar Province.
The weapon weighs a staggering 21,600 pounds.
It is known as the 'Mother Of All Bombs' – a play on 'MOAB,' an acronym that stands for 'Massive Ordnance Air Burst.'
A crater left by the blast is believed to be more than 300 meters (1,000 feet) wide after it exploded six feet above the ground. Anyone at the blast site was vaporized.
President Donald Trump told reporters at the White House that he was 'very, very proud' and called the operation 'really another successful job. We're very, very proud of our military.'