The wrestler and model was a WWE Superstar from 2005 to 2008, and rose to even greater notoriety among a mainstream audience after appearing on the cover of Playboy.
The WWE organization released a statement in response to Massaro's death, offering their condolences to the fighter's family and friends.
'We are saddened to learn of the tragic death of former WWE Superstar Ashley Massaro,' the statement read.
Massaro had one daughter, Alexis, who will turn 19 years old in July.
The Suffolk County Medical Examiner's office told DailyMail.com a spokesperson would not be available until Friday morning to answer questions related to Massaro's cause of death.